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Center for Cellular Nanoanalytics Osnabrück


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Selected Publications

2022

Ca2+-activated sphingomyelin scrambling and turnover mediate ESCRT-independent lysosomal repair.

Niekamp P, Scharte F, Sokoya T, Vittadello L, Kim Y, Deng Y, Südhoff E, Hilderink A, Imlau M, Clarke CJ, Hensel M, Burd CG, Holthuis JCM (2022) Nature Communications 13:1875,

Compartmentation and functions of sphingolipids

Körner C, Fröhlich F (2022) Curr Opin Cell Biol. 74:104-111.

Structure of the Mon1-Ccz1 complex reveals molecular basis of membrane binding for Rab7 activation

Klink B, Herrmann E, Antoni C, Langemeyer L, Kiontke S, Gatsogiannis C, Ungermann C, Raunser S, Kümmel D (2022) Proc Natl Acad Sci U S A. 119(6):e2121494119.

Monitoring and quantification of the dynamics of sress granule components in living cells by fluorescence decay after photoactivation

Söhnel AC, Trushina NI, Brandt R (2022) Methods Mol Biol. 2428:243-259.

A lysosomal biogenesis map reveals the cargo spectrum of yeast vacuolar protein targeting pathways

Eising S, Esch B, Wälte M, Vargas Duarte P, Walter S, Ungermann C, Bohnert M, Fröhlich F (2022) J Cell Biol. 221(4):e202107148.

The yeast LYST homolog Bph1 is a Rab5 effector and prevents Atg8 lipidation at endosomes

Vargas Duarte P,  Hardenberg R, Mari M, Walter S, Reggiori F, Fröhlich F, González Montoro A, Ungermann C (2022) J Cell Sci. 135(8):jcs259421

Intracellular Salmonella Paratyphi A is motile and differs in the expression of flagella-chemotaxis, SPI-1 and carbon utilization pathways in comparison to intracellular S. Typhimurium

Cohen H, Hoede C, Scharte F, Coluzzi C, Cohen E, Shomer I, Mallet L, Holbert S, Serre RF,  Schiex T, Virlogeux-Payant I, Grassl GA, Hensel M, Chiapello H, Gal-Mor O (2022) PLoS Pathog. 18(4):e1010425.

Dissociation of β2m from MHC class I triggers formation of noncovalent, transient heavy chain dimers

Dirscherl C, Löchte S, Hein Z, Kopicki JD, Harders AR, Linden N, Karner A, Preiner J, Weghuber J, Garcia-Alai M, Uetrecht C, Zacharias M, Piehler J, Lanzerstorfer P, Springer S (2022) J Cell Sci. jcs.259498

The HOPS tethering complex is required to maintain signaling endosome identity and TORC1 activity

Gao J, Nicastro R, Péli-Gulli M-P, Grziwa S, Chen Z, Kurre R, Piehler J, De Virgilio C, Fröhlich F, Ungermann C (2022) J Cell Biol  221(5):e202109084.

Four-color single-molecule imaging with engineered tags resolves the molecular architecture of signaling complexes in the plasma membrane

Bellón JS, Birkholz O, Richter CP, Eull F, Kenneweg H, Wilmes S, Rothbauer U, You C, Walter MR, Kurre R, Piehler J. (2022) Cell Reports Methods 2(2) 100165

Surface modification of polycaprolactone scaffold with improved biocompatibility and controlled growth factor release for enhanced stem cell differentiation

Qin X, Wu Y, Liu S, Yang L, Yuan H, Cai S, Flesch J, Li Z, Tang Y, Li X, Zhuang Y, You C, Liu C, Yu C. Front Bioeng Biotechnol (2022) Jan 7;9:802311.

Frozen motion: how cryo-EM changes the way we look at ABC transporters

Shvarev D, Januliene D, Moeller A. Trends Biochem Sci (2022) Feb;47(2):136-148.

Low membrane fluidity triggers lipid phase separation and protein segregation in living bacteria

Gohrbandt M, Lipski A, Grimshaw JW, Buttress JA, Baig Z, Herkenhoff B, Walter S, Kurre R, Deckers-Hebestreit G, Strahl H. EMBO J (2022) Jan 17;e109800.

Systematic Assessment of the Accuracy of Subunit Counting in Biomolecular Complexes Using Automated Single-Molecule Brightness Analysis

Danial JSH, Quintana Y, Ros U, Shalaby R, Margheritis EG, Chumpen Ramirez S, Ungermann C, Garcia-Saez AJ, Cosentino K. J Phys Chem Lett (2022) Jan 27;13(3):822-829

2021

Ischemic tolerance and cardiac repair in the spiny mouse (Acomys)

Koopmans T, van Beijnum H, Roovers EF, Tomasso A, Malhotra D, Boeter J, Psathaki OE, Versteeg D, van Rooij E, Bartscherer K. NPJ Regen Med (2021) 6(1),78.

Single cell analyses reveal distinct adaptation of typhoidal and non-typhoidal Salmonella enterica serovars to intracellular lifestyle

Röder J, Felgner P, Hensel M. Cell Microbiol (2021) 23(10):e13374.

Micro-Meta App: an interactive tool for collecting microscopy metadata based on community specifications

Rigano A, Ehmsen S, Öztürk SU, Ryan J, Balashov A, Hammer M, Kirli K, Boehm U, Brown CM, Bellve K, Chambers JJ, Cosolo A, Coleman RA, Faklaris O, Fogarty KE, Guilbert T, Hamacher AB, Itano MS, Keeley DP, Kunis S, Lacoste J, Laude A, Ma WY, Marcello M, Montero-Llopis P, Nelson G, Nitschke R, Pimentel JA, Weidtkamp-Peters S, Park PJ, Alver BH, Grunwald D, & Strambio-De-Castillia C
Nat Methods (2021) Dec;18(12):1489-1495

Subunit exchange among endolysosomal tethering complexes is linked to contact site formation at the vacuole

Gonzáles Montoro A, Vargas Duarte P, Auffarth K, Walter S, Fröhlich F, & Ungermann C
Mol Biol Cell (2021) Dec 1;32(22):br14. doi: 10.1091/mbc.E21-05-0227

IFNAR1 and IFNAR2 play distinct roles in initiating type I interferon-induced JAK-STAT signaling and activating STATs

Shemesh M, Lochte S, Piehler J, & Schreiber G
Sci Signal (2021) Nov 23;14(710):eabe4627

Subunit exchange among endolysosomal tethering complexes is linked to contact site formation at the vacuole

González Montoro A, Duarte PV, Auffarth K, Walter S, Fröhlich F, Ungermann C
Mol Biol Cell (2021) Nov 18 32(22) mbc.E21-05-0227

Metabolic dissimilarity determines the establishment of cross-feeding interactions in bacteria

Giri S, Oña L, Waschina S, Shitut S, Yousif G, Kaleta C, Kost C
Curr Biol. (2021) Oct 27;S0960-9822(21)01408-1

Dendrite tapering actuates a self-organizing signaling circuit for stochastic filopodia initiation in neurons

Mancinelli G, Lamparter L, Nosov G, Saha T,  Pawluchin A, Kurre R, Rasch C, Ebrahimkutty M, Klingauf J, & Galic M
Proc Natl Acad Sci U S A (2021) Oct 26;118(43):e2106921118

Flexible open conformation of the AP-3 complex explains its role in cargo recruitment at the Golgi

Schoppe J, Schubert E, Apelbaum A, Yavavli E, Birkholz O, Stephanowitz H, Han Y, Perz A, Hofnagel O, Liu F, Piehler J, Raunser S, Ungermann C
J Biol Chem. (2021) 101334

A reference scale of cucurbit[7]uril binding affinities

Alnajja MA, Nau WM, & Hennig A
Org Biomol Chem (2021) Oct 14;19(39):8521-8529

Diffraction-Unlimited Photomanipulation at the Plasma Membrane via Specifically Targeted Upconversion Nanoparticles

Drees C, Rühl P, Czerny J, Chandra G, Bajorath J, Haase M, Heinemann SH, Piehler J
Nano Lett. (2021) Oct 13;21(19):8025-8034.

MDEmic: a metadata annotation tool to facilitate management of FAIR image data in the bioimaging community

Kunis S, Hänsch S, Schmidt C, Wong F, Strambio-De-Castillia C, Weidtkamp-Peters S
Nature Methods (2021) Oct 11 doi.org/10.1038/s41592-021-01288-z

QUAREP-LiMi: A community-driven initiative to establish guidelines for quality assessment and reproducibility for instruments and images in light microscopy

Nelson G, Boehm U, Bagley S, Bajcsy P, Bischof J, Brown CM, Dauphin A, Dobbie IM, Eriksson JE, Faklaris O, Fernandez-Rodriguez J, Ferrand A, Gelman L, Gheisari A, Hartmann H, Kukat C, Laude A, Mitkovski M, Munck S, North AJ, Rasse TM, Resch-Genger U, Schuetz LC, Seitz A, Strambio-De-Castillia C, Swedlow JR, Alexopoulos I, Aumayr K, Avilov S, Bakker G-J, Bammann RR, Bassi A, Beckert H, Beer S, Belyaev Y, Bierwagen J, Birngruber KA, Bosch M, Breitlow J, Cameron LA, Chalfoun J, Chambers JJ, Chen C-L, Conde-Sousa E, Corbett AD, Cordelieres FP, Del Nery E, Dietzel R, Eismann F, Fazeli E, Felscher A, Fried H, Gaudreault N, Goh WI , Guilbert T, Hadleigh R, Hemmerich P, Holst GA, Itano MS, Jaffe CB, Jambor HK, Jarvis SC, Keppler A, Kirchenbuechler D, Kirchner M, Kobayashi N, Krens G, Kunis S, Lacoste J, Marcello M, Martins GG, Metcalf DJ, Mitchell CA, Moore J, Mueller T, Nelson MS, Ogg S, Onami S, Palmer AL, Paul-Gilloteaux P, Pimentel JA, Plantard L, Podder S, Rexhepaj E, Royon A, Saari MA, Schapman D, Schoonderwoert V,  Schroth-Diez B, Schwartz S, Shaw M, Spitaler M, Stoeckl MT, Sudar D, Teillon J, Terjung S, Thuenauer R, Wilms CD, Wright GD, & Nitschke R
J Microsc (2021) Oct;284(1):56-73. 

Flexible open conformation of the AP-3 complex explains its role in cargo recruitment at the Golgi

Schoppe J, Schubert E, Apelbaum A, Yavavli E, Birkholz O, Stephanowitz H, Han Y, Perz A, Hofnagel O, Liu F, Piehler J, Raunser S, & Ungermann C
J Biol Chem 297 (2021), 101334

The small GTPase KlRho5 responds to oxidative stress and affects cytokinesis

Musielak M, Sterk CC, Schubert F, Meyer C, Paululat A, Heinisch JJ
J Cell Sci. (2021), Sep 15;134(18):jcs258301

Obligate cross-feeding expands the metabolic niche of bacteria

Oña L, Giri G, Avermann N, Kreienbaum M, Thormann KM, Kost C
Nat Ecol Evol. (2021) Sep;5(9):1224-1232

TSC1 binding to lysosomal PIPs is required for TSC complex translocation and mTORC1 regulation

Fitzian K, Brückner A, Brohée L, Zech R, Antoni C, Kiontke S, Gasper R, Linard Matos AL, Beel S, Wilhelm S, Gerke V, Ungermann C, Nellist M, Raunser S, Demetriades C, Oeckinghaus A, & Kümmel D
Mol Cell (2021), Jul 1 81(13):2705-2721

Who’s in control? Principles of Rab GTPase activation in endolysosomal membrane trafficking and beyond

Borchers AC, Langemeyer L, & Ungermann C
J Cell Biol (2021) 220 (9): e202105120

Apoptosis-inducing anti-HER2 agents operate through oligomerization-induced receptor immobilization

Stüber JC, Richter CP, Sotolongo Bellón J, Schwill M, König I, Schuler B, Piehler J, Plückthun A
Commun Biol, (2021) Jun 21;4(1):762

In-vivo tracking of harmonic nanoparticles: a study based on a TIGER widefield microscope

Vittadello L, Kijatkin C, Klenen J, Dzikonski D, Kömpe K, Meyer C, Paululat A, Imlau M
Optical Materials Express, (2021) 11, 1953-1969

Engineering an anti-HER2 biparatopic antibody with a multimodal mechanism of action

Kast F, Schwill M, Stüber JC, Pfundstein S, Nagy-Davidescu G, Rodríguez JMM, Seehusen F, Richter CP, Honegger A, Hartmann KP, Weber TG, Kroener F, Ernst P, Piehler J, Plückthun A
Nat Commun, (2021) Jun 18;12(1):3790

Evaluation of DIBMA nanoparticles of variable size and anionic lipid content as tools for the structural and functional study of membrane proteins

Voskoboynikova N, Margheritis EG, Kodde F, Rademacher M, Schowe M, Budke-Gieseking A, Psathaki OE, Steinhoff HJ, Cosentino K
Biochim Biophys Acta Biomembr, (2021) Jun 1;1863(6):183588

TSC1 binding to lysosomal PIPs is required for TSC complex translocation and mTORC1 regulation

Fitzian K, Brückner A, Brohée L, Zech R, Antoni C, Kiontke S, Gasper R, Linard Matos AL, Beel S, Wilhelm S, Gerke V, Ungermann C, Nellist M, Raunser S, Demetriades C, Oeckinghaus A, Kümmel D
Mol Cell, (2021) Apr 30:S1097-2765(21)00324-5

Lipid Dynamics in Diisobutylene-Maleic Acid (DIBMA) Lipid Particles in Presence of Sensory Rhodopsin II

Voskoboynikova N, Orekhov P, Bozdaganyan M, Kodde F, Rademacher M, Schowe M, Budke-Gieseking A, Brickwedde B, Psathaki OE, Mulkidjanian AY, Cosentino K, Shaitan KV, Steinhoff HJ
Int J Mol Sci, (2021) Mar 4;22(5):2548

The protected physiological state of intracellular Salmonella enterica persisters reduces host cell-imposed stress

Schulte M, Olschewski K, Hensel M
Commun Biol, (2021) May 4;4(1):520

Calibration of cell-intrinsic interleukin-2 response thresholds guides design of a regulatory T cell biased agonist

Glassman CR, Su L, Majri-Morrison SS, Winkelmann H, Mo F, Li P, Pérez-Cruz M, Ho PP, Koliesnik I, Nagy N, Hnizdilova T, Picton LK, Kovar M, Bollyky P, Steinman L, Meyer E, Piehler J, Leonard WJ, Garcia KC
Elife, (2021) May 18;10:e65777

Biofunctionalization of carbon nanotubes for reversible site-specific protein immobilization

Jorde L, Li Z, Pöppelwerth A, Piehler J, You C, Meyer C
Journal of Applied Physics, (2021) 129, 094302

Competitive binding of STATs to receptor phospho-Tyr motifs accounts for altered cytokine responses

Wilmes S, Jeffrey PA, Martinez-Fabregas J, Hafer M, Fyfe PK, Pohler E, Gaggero S, López-García M, Lythe G, Taylor C, Guerrier T, Launay D, Mitra S, Piehler J, Molina-París C, Moraga I
Elife, (2021) Apr 19;10:e66014

TSC1 binding to lysosomal PIPs is required for TSC complex translocation and mTORC1 regulation

Fitzian K, Brückner A, Brohée L, Zech R, Antoni C, Kiontke S, Gasper R, Linard Matos AL, Beel S, Wilhelm S, Gerke V, Ungermann C, Nellist M, Raunser S, Demetriades C, Oeckinghaus A, Kümmel D
Mol Cell, (2021) Apr 30:S1097-2765(21)00324-5

Fluorescent protein-based reporters reveal stress response of intracellular Salmonella enterica at level of single bacterial cells

Schulte M, Olschewski K, Hensel M
Cell Microbiol, (2021) Mar;23(3):e13293

n-type Ge/Si antennas for THz sensing

Chavarin CA, Hardt E, Gruessing S, Skibitzki O, Costina I, Spirito D, Seifert W, Klesse W, Manganelli CL, You C, Flesch J, Piehler J, Missori M, Baldassarre L, Witzigmann B, Capellini G
Opt Express, (2021) Mar 1;29(5):7680-7689

Correlative Light and Scanning Electron Microscopy to Study Interactions of Salmonella enterica with Polarized Epithelial Cell Monolayers

Kommnick C, Hensel M
Methods Mol Biol, (2021) J2182:103-115

Three-Dimensional Interfacing of Cells with Hierarchical Silicon Nano/Microstructures for Midinfrared Interrogation of In Situ Captured Proteins

Flesch J, Bettenhausen M, Kazmierczak M, Klesse WM, Skibitzki O, Psathaki OE, Kurre R, Capellini G, Guha S, Schroeder T, Witzigmann B, You C, Piehler J
ACS Appl Mater Interfaces, (2021) Feb 11

Self-Labeling Enzyme Tags for Translocation Analyses of Salmonella Effector Proteins

Göser V, Hensel M
Methods Mol Biol, (2021) 2182:67-82

Short Photoswitchable Ceramides Enable Optical Control of Apoptosis

Morstein J, Kol M, Novak AJE, Feng S, Khayyo S, Hinnah K, Li-Purcell N, Pan G, Williams BM, Riezman H, Atilla-Gokcumen GE, Holthuis JCM, Trauner D
ACS Chem Biol, (2021) Mar 19;16(3):452-456

A Three-Dimensional Model of the Yeast Transmembrane Sensor Wsc1 Obtained by SMA-Based Detergent-Free Purification and Transmission Electron Microscopy

Voskoboynikova N, Karlova M, Kurre R, Mulkidjanian AY, Shaitan KV, Sokolova OS, Steinhoff HJ, Heinisch JJ
J Fungi (Basel), (2021) Feb 5;7(2):118

Zn\(^{2+}\) Intoxication of Mycobacterium marinum during Dictyostelium discoideum Infection Is Counteracted by Induction of the Pathogen Zn\(^{2+}\) Exporter CtpC

Hanna N, Koliwer-Brandl H, Lefrançois LH, Kalinina V, Cardenal-Muñoz E, Appiah J, Leuba F, Gueho A, Hilbi H, Soldati T, Barisch C
mBio, (2021) Feb 2;12(1):e01313-20

Nanoscopic anatomy of dynamic multi-protein complexes at membranes resolved by graphene-induced energy transfer

Füllbrunn N, Li Z, Jorde L, Richter CP, Kurre R, Langemeyer L, Yu C, Meyer C, Enderlein J, Ungermann C, Piehler J, You C
Elife, (2021) Jan 29;10:e62501

Sphingomyelin Biosynthesis Is Essential for Phagocytic Signaling during Mycobacterium tuberculosis Host Cell Entry

Niekamp P, Guzman G, Leier HC, Rashidfarrokhi A, Richina V, Pott F, Barisch C, Holthuis JCM, Tafesse FG
mBio, (2021) Jan 26;12(1):e03141-20

TORC1 Determines Fab1 Lipid Kinase Function at Signaling Endosomes and Vacuoles

Chen Z, Malia PC, Hatakeyama R, Nicastro R, Hu Z, Péli-Gulli MP, Gao J, Nishimura T, Eskes E, Stefan CJ, Winderickx J, Dengjel J, De Virgilio C, Ungermann C
Curr Biol,(2021) Jan 25;31(2):297-309.e8

Membrane Permeability and Its Activation Energies in Dependence on Analyte, Lipid, and Phase Type Obtained by the Fluorescent Artificial Receptor Membrane Assay

Nilan M, Collin S, Karmacharya S. Hennig A, & Nau WM
ACS Sens (2021) Jan 22;6(1):175-182

Tuning MPL signaling to influence hematopoietic stem cell differentiation and inhibit essential thrombocythemia progenitors

Cui L, Moraga I, Lerbs T, Van Neste C, Wilmes S, Tsutsumi N, Trotman-Grant AC, Gakovic M, Andrews S, Gotlib J, Darmanis S, Enge M, Quake S, Hitchcock IS, Piehler J, Garcia KC, Wernig G
Proc Natl Acad Sci USA, (2021) Jan 12;118(2):e2017849118 (2021)

Capillary Stamping of Functional Materials: Parallel Additive Substrate Patterning without Ink Depletion

Runge M, Hübner H, Grimm A, Manoharan G, Wieczorek R, Philippi M, Harneit W, Meyer C, Enke D, Gallei M, Steinhart M
Advanced Materials Interfaces, (2021) 8, 2001911

2020

Interaction of Cucurbit[7]uril with protease substrates: application to nanosecond time-resolved fluorescence assays

Hennig A, Nau WM
Front Chem (2020) Sep 10

Parallelized Manipulation of Adherent Living Cells by Magnetic Nanoparticles-Mediated Forces

Bongaerts M, Aizel K, Secret E, Jan A, Nahar T, Raudzus F, Neumann S, Telling N, Heumann R, Siaugue JM, Menager C, Fresnais J, Villard C, El Haj A, Piehler J, Gates MA, & Coppey M
International journal of molecular sciences, 21 (2020)

Na1.5-x/2Gd1.5-x/2SrxF6 nanocrystals: Growth, size control and shell formation on β-NaCeF\(_{4}\):Tb core particles

Wehmeier J, Haase M
CrystEngComm, (2020) 22, 8036-8044

Engineered IL-10 variants elicit potent immunomodulatory effects at low ligand doses

Gorby C, Sotolongo Bellon J, Wilmes S, Warda W, Pohler E, Fyfe PK, Cozzani A, Ferrand C, Walter MR, Mitra S, Piehler J, & Moraga I
Sci Signal, 13, 649 (2020)

Towards a mathematical model for single molecule structured illumination microscopy

Hockmann M, Kunis S, and Kurre R
Proc Appl Math Mech, (2020)

Function of the SNARE Ykt6 on autophagosomes requires the Dsl1 complex and the Atg1 kinase complex

Gao J, Kurre R, Rose J, Walter S, Fröhlich F, Piehler J, Reggiori F, Ungermann C
EMBO Rep, (2020) Oct 7:e50733

Magnetic and Electronic Properties of Highly Mn-Doped β-NaGdF\(_{4}\) and β-NaEuF\(_{4}\) Nanoparticles with a Narrow Size Distribution

Schneider L, Wehmeier J, Wiedwald U, Rodewald J, Galakhov VR, Udintseva MS, Mesilov V, Radu F, Luo C, Klare JP, Steinhoff H-J, Haase M, Kuepper K
J Phys Chem C, (2020) 124, 33, 18194–18202

On the condition number of Vandermonde matrices with pairs of nearly-colliding nodes

Kunis S and Nagel D
Numer Algorithms, (2020)

On the smallest singular value of multivariate Vandermonde matrices with clustered nodes

Kunis S and Nagel D
Linear Algebra Appl, (2020) 604, 1-20

Size-Controlled Growth of β-NaGdF4 and β-NaGdF\(_{4}\):Yb,Er Nanocrystals: The Influence of the Surface Area of NaF on the Nucleation of the β-Phase

Czerny J, Heil F, Egbers CJ, Haase M
Chemistry of Materials, (2020) 32 (13), 5691-5699

Heme binding of transmembrane signaling proteins undergoing regulated intramembrane proteolysis

Kupke T, Klare JP, Brügger B
Commun Biol, (2020) Feb 14;3(1):73

Learning algebraic decompositions using Prony structures

Kunis S, Römer T, and von der Ohe U
Adv in Appl Math, (2020) 118, 102044

LiYF\(_{4}\):Yb/LiYF\(_{4}\): and LiYF\(_{4}\)::Yb,Er/LiYF\(_{4}\): core/shell nanocrystals with luminescence decay times similar to YLF laser crystals and the upconversion quantum yield of the Yb,Er doped nanocrystals

Carl F, Birk L, Grauel B, Pons M, Würth C, Resch-Genger U, Haase M
Nano Research, (2020)

A conserved and regulated mechanism drives endosomal Rab transition

Langemeyer L, Borchers AC, Herrmann E, Füllbrunn N, Han Y, Perz A, Auffarth K, Kümmel D, Ungermann C
Elife, (2020) 9:e56090

Time‐Resolved Nonlinear Diffuse fs‐Pulse Reflectometry Using Lithium Niobate Nanoparticles with Two Pulses of Different Colors

Kijatkin C, Bourdon B, Klenen J, Kocsor L, Szaller Z, Imlau M
Adv Photonics Res, (2020) Sep 29

Dual-color 3D-dSTORM colocalization and quantification of ROXY1 and RNAPII variants throughout the transcription cycle in root meristem nuclei

Maß L, Holtmannspötter M, Zachgo S
Plant J, (2020) Sep 8

The role of cations in hydrothermal synthesis of nonlinear optical sodium niobate nanocrystals

Kohlenbach ND, Kijatkin C, König M, Haase M, Imlau M, Kömpe K
Nanoscale, (2020) Sep 15

AP‐3 vesicle uncoating occurs after HOPS‐dependent vacuole tethering

Schoppe J, Mari M, Yavavli E, Auffarth K, Cabrera M, Walter S, Fröhlich F, Ungermann C
EMBO J, (2020) Aug 25:e105117

Next-Generation Surrogate Wnts Support Organoid Growth and Deconvolute Frizzled Pleiotropy In Vivo

Miao Y, Ha A, de Lau W, Yuki K, Santos AJM, You C, Geurts MH, Puschhof J, Pleguezuelos-Manzano C, Peng WC, Senlice R, Piani C, Buikema JW, Gbenedio OM, Vallon M, Yuan J, de Haan S, Hemrika W, Rösch K, Dang LT, Baker D, Ott M, Depeille P, Wu SM, Drost J, Nusse R, Roose JP, Piehler J, Boj SF, Janda CY, Clevers H, Kuo CJ, Garcia KC
Cell Stem Cell, (2020) S1934-5909(20)30358-1

In cellulo protein semi‐synthesis from endogenous and exogenous fragments using the ultra‐fast split Gp41‐1 intein

Bhagawati M, Hoffmann S, Höffgen KS, Piehler J, Busch K, Mootz HD
Angew Chem Int Ed Engl, (2020) Aug 10

In cell Gd\(^{3+}\)-based site-directed spin labeling and EPR spectroscopy of eGFP

Kucher S, Korneev S, Klare JP, Klose D, Steinhoff HJ
Phys Chem Chem Phys, (2020) 22(24):13358-13362

Live imaging of intra-lysosome pH in cell lines and primary neuronal culture using a novel genetically encoded biosensor

Ponsford AH, Ryan TA, Raimondi A, Cocucci E, Wycislo SA, Fröhlich F, Swan LE, Stagi M
Autophagy, (2020) Jun 9:1-19

Lipid dynamics in nanoparticles formed by maleic acid-containing copolymers: EPR spectroscopy and molecular dynamics simulations

Colbasevici A, Voskoboynikova N, Orekhov PS, Bozdaganyan ME, Karlova MG, Sokolova OS, Klare JP, Mulkidjanian AY, Shaitan KV, Steinhoff HJ
Biochim Biophys Acta Biomembr, (2020) 1862(5):183207

Uptake of exogenous serine is important to maintain sphingolipid homeostasis in Saccharomyces cerevisiae

Esch BM, Limar S, Bogdanowski A, Gournas C, More T, Sundag C, Walter S, Heinisch JJ, Ejsing CS, André B, Fröhlich F
PLoS Genet, (2020) 16(8):e1008745

A trimeric metazoan Rab7 GEF complex is crucial for endocytosis and scavenger function

Dehnen L, Janz M, Verma JK, Psathaki OE, Langemeyer L, Fröhlich F, Heinisch JJ, Meyer H, Ungermann C, Paululat A
Journal of Cell Science, (2020) 4133(13):jcs247080

The spatio-temporal organization of mitochondrial F\(_{1}\)F\(_{0}\)ATP synthase in cristae depends on its activity mode

Salewskij K, Rieger B, Hager F, Arroum T, Duwe P, Villalta J, Colgiati S, Richter CP, Psathaki OE, Enriquez JA, Dellmann T, Busch KB
Biochim Biophys Acta Bioenerg, (2020) 1861(1):148091

A trafficome-wide RNAi screen reveals deployment of early and late secretory host proteins and the entire late endo-/lysosomal vesicle fusion machinery by intracellular Salmonella

Kehl A, Göser V, Reuter T, Liss V, Franke M , John C, Richter CP, Deiwick J, Hensel M
PLoS Pathog, (2020) 16(7):e1008220

Novel Fluorescent Mitochondria-Targeted Probe MitoCLox Reports Lipid Peroxidation in Response to Oxidative Stress In Vivo

Lyamzaev KG, Panteleeva AA, Karpukhina AA, Galkin II, Popova EN, Pletjushkina OY, Rieger B, Busch KB, Mulkidjanian AY, Chernyak BV
Oxid Med Cell Longev, (2020) 3631272

Self-assembly of robust gold nanoparticle monolayer architectures for quantitative protein interaction analysis by LSPR spectroscopy

Flesch J, Kappen M, Drees C, You C, Piehler J
Analytical and Bioanalytical Chemistr, (2020) 412:3413–3422

Type I IFN is siloed in endosomes

Altman JB, Taft J, Wedeking T, Gruber CN, Holtmannspötter M, Piehler J, and Bogunovic D,
PNAS, (2020) 30, 17510-17512

Mechanism of homodimeric cytokine receptor activation and dysregulation by oncogenic mutations

Wilmes S, Hafer M, Vuorio J, Tucker JA, Winkelmann H, Löchte S, Stanly TA, Pulgar Prieto KD , Poojari C, Sharma V, Richter CP, Kurre R, Hubbard SR, Garcia KC, Moraga I, Vattulainen I, Hitchcock IS, Piehler J
Science, 367(6478), 643-652 (2020)

2019

Kinetics of cytokine receptor trafficking determine signaling and functional selectivity

Martinez-Fabregas J, Wilmes S, Wang L, Hafer M, Pohler E, Lokau J, Garbers C, Cozzani A, Fyfe PK, Piehler J, Kazemian M, Mitra S, Moraga I
Elife, (2019) Nov 27;8:e49314

Prony's method on the sphere

Kunis S, Möller HM, and von der Ohe U
SMAI J Comput Math, (2019) 5S, 87-97

Structure of the IFNγ receptor complex guides design of biased agonists

Mendoza JL, Escalante NK, Jude KM, Sotolongo Bellon J, Su L, Horton TM, Tsutsumi N, Berardinelli SJ, Haltiwanger RS, Piehler J, Engleman EG, Garcia KC
Nature, (2019) Mar;567(7746):56-60

A randomized multivariate matrix pencil method for superresolution microscopy

Ehler M, Kunis S, Peter T, and Richter C
Electron Trans Numer Anal, (2019) . 51, 63-74

MpTCP1 controls cell proliferation and redox processes in Marchantia polymorpha

Busch A, Deckena M, Almeida-Trapp M, Kopischke S, Kock C, Schüssler E, Tsiantis M, Mithöfer A, Zachgo S
New Phytol, (2019) Dec;224(4):1627-1641

Close-packed silane nanodot arrays by capillary nanostamping coupled with heterocyclic silane ring opening

Philippi M, You C, Richter CP, Schmidt M, Thien J, Liße D, Wollschlager J, Piehler J and Steinhart M
RSC Adv, 2019, 9, 24742

Blocks in Tricarboxylic Acid Cycle of Salmonella enterica Cause Global Perturbation of Carbon Storage, Motility, and Host-Pathogen Interaction

Noster J, Hansmeier N, Persicke M, Chao TC, Kurre R, Popp J, Liss V, Reuter T, Hensel M
mSphere, (2019) 4(6):e00796-19

Topological control of cytokine receptor signaling induces differential effects in hematopoiesis

Mohan K, Ueda G, Kim AR, Jude KM, Fallas JA, Guo Y, Hafer M, Miao Y, Saxton RA, Piehler J, Sankaran VG, Baker D, Garcia KC
Science, (2019) May 24;364(6442):eaav7532

MitoCLox: A Novel Mitochondria-Targeted Fluorescent Probe for Tracing Lipid Peroxidation

Konstantin G, Lyamzaev KG, Sumbatyan NV, Nesterenko AM, Kholina EG, Voskoboynikova N, Steinhoff H-J, Mulkidjanian AY
Oxid Med Cell Longev, (2019) 9710208

ESEEM Reveals Bound Substrate Histidine in the ABC Transporter HisQMP\(_2\)

Isaev N, Heuveling J, Ivanisenko N, Schneider E, Steinhoff HJ
Applied Magnetic Resonance, 1-11 (2019)

Conformational dynamics of sensory rhodopsin II in nanolipoprotein and styrene-maleic acid lipid particles

Mosslehy W, Voskoboynikova N, Colbasevici A, Ricke A, Klose D, Klare JP, Mulkidzhanyan AY, Steinhoff HJ
Photochem Photobiol, (2019)

Proton leakage across lipid bilayers: Oxygen atoms of phospholipid ester linkers align water molecules into transmembrane water wires

Bozdaganyan ME, Lokhmatikov AV, Voskoboynikova N, Cherepanov DA, Steinhoff HJ, Shaitan KV, Mulkidjanian AY
BBA-Bioenergetics 1860(6), 439-451 (2019)

Styrene/Maleic Acid Copolymers Form SMALPs by Pulling Lipid Patches out of the Lipid Bilayer

Orekhov PS, Bozdaganyan ME, Voskoboynikova N, Mulkidjanian AY, Steinhoff HJ, Shaitan KV
Langmuir 35(10), 3748-3758 (2019)

Detergent-free solubilization of human Kv channels expressed in mammalian cells

Karlova MG, Voskoboynikova N, Gluhov GS, Abramochkin D, Malak OA, Mulkidzhanyan A, Loussouarn G, Steinhoff HJ, Shaitan KV, Sokolova OS
Chem Phys Lipids 219 (2019)

Optical manipulation of sphingolipid biosynthesis using photoswitchable ceramides

Kol M, Williams B, Toombs-Ruane H, Franquelim HG, Korneev S, Schroeer C, Schwille P, Trauner D, Holthuis JC, Frank JA
Elife, 8:e43230 (2019)

Osteoporosis and skeletal dysplasia caused by pathogenic variants in SGMS2

Pekkinen M, Terhal PA, Botto LD, Henning P, Mäkitie RE, Roschger P, Jain A, Kol M, Kjellberg MA, Paschalis EP, van Gassen K, Murray M, Bayrak-Toydemir P, Magnusson MK, Jans J, Kausar M, Carey JC, Somerharju P, Lerner UH, Olkkonen VM, Klaushofer K, Holthuis JC, Mäkitie O
JCI Insight, 4(7):e126180 (2019)

Ceramides bind VDAC2 to trigger mitochondrial apoptosis

Dadsena S, Bockelmann S, Mina JGM, Hassan DG, Korneev S, Razzera G, Jahn H, Niekamp P, Müller D, Schneider M, Tafesse FG, Marrink SJ, Melo MN, Holthuis JCM
Nat Commun, 10(1), 1832 (2019)

A systematic approach to identify recycling endocytic cargo depending on the GARP complex

Eising S, Thiele L, Fröhlich F
eLife 8:e42837 (2019)

2018

Annexins A2 and A6 interact with the extreme N terminus of tau and thereby contribute to tau's axonal localization

Gauthier-Kemper A, Suárez Alonso M, Sündermann F, Niewidok B, Fernandez MP, Bakota L, Heinisch JJ, Brandt R
J Biol Chem, (2018) May 25;293(21):8065-8076

Identification and In Vivo Characterisation of Cardioactive Peptides in Drosophila melanogaster

Schiemann R, Lammers K, Janz M, Lohmann J, Paululat A, Meyer H
Int J Mol Sci., (2018) Dec 20;20(1):2

SERCA is critical to control the Bowditch effect in the heart

Balcazar D, Regge V, Santalla M, Meyer H, Paululat A, Mattiazzi A, Ferrero P
Sci Rep, (2018) 8, 12447

APC/C\(^{Fzr}\) regulates cardiac and myoblast cell numbers, and plays a crucial role during myoblast fusion

Drechsler M, Meyer H, Wilmes AC, Paululat A
J Cell Sci, (2018) Jul 17;131(14):jcs209155

Drosophila pericardial nephrocyte ultrastructure changes during ageing

Psathaki OE, Dehnen L, Hartley PS, Paululat A
Mech Ageing Dev, (2018) 173:9-20

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Appelhans T, Beinlich FRM, Richter CP, Kurre R, Busch KB
J Vis Exp., (2018) 136:57690

Single-molecule imaging reveals dynamic biphasic partition of RNA-binding proteins in stress granules

Niewidok B, Igaev M, Pereira da Graca A, Strassner A, Lenzen C, Richter CP, Piehler J, Kurre R, Brandt R
J Cell Biol, 217, 1303-1318 (2018)

Stable and selective self-assembly of α-lipoic acid on Ge(001) for biomolecule immobilization

Kazmierczak M, Flesch J, Mitzloff J, Capellini G, Klesse WM, Skibitzki O, You C, Bettenhausen M, Witzigmann B, Piehler J, Schroeder T, Guha S
Journal of Applied Physics, 123, 175305 (2018)

Non-specific interactions govern cytosolic diffusion of nanosized objects in mammalian cells

Etoc F, Balloul E, Vicario C, Normanno D, Liße D, Sittner A, Piehler J, Dahan M, Coppey M
Nature materials, 17, 740-746 (2018)

How nanoscale protein interactions determine the mesoscale dynamic organisation of bacterial outer membrane proteins

Chavent M, Duncan AL, Rassam P, Birkholz O, Hélie J, Reddy T, Beliaev D, Hambly B, Piehler J, Kleanthous C, Sansom MSP
Nat Commun, 9, 2846 (2018)

Multi-functional DNA nanostructures that puncture and remodel lipid membranes into hybrid materials

Birkholz O, Burns JR, Richter CP, Psathaki OE, Howorka S, Piehler J
Nat Commun, 9, 1521 (2018)

Germanium Plasmon Enhanced Resonators for Label-Free Terahertz Protein Sensing

Bettenhausen M, Römer F, Witzigmann B, Flesch J, Kurre R, Korneev S, Piehler J, You C, Kazmierczak M, Guha S, Capellini G, Schröder T
Frequenz, 72, 113-122 (2018)

Near-native, site-specific and purification-free protein labeling for quantitative protein interaction analysis by MicroScale Thermophoresis

Bartoschik T, Galinec S, Kleusch C, Walkiewicz K, Breitsprecher D, Weigert S, Muller YA, You C, Piehler J, Vercruysse T, Daelemans D, Tschammer N
Scientific reports, 8, 4977 (2018)

A search for ceramide binding proteins using bifunctional lipid analogues yields CERT-related protein StarD7

Bockelmann S, Mina JGM, Korneev S, Hassan DG, Müller D, Hilderink A, Vlieg HC, Raijmakers R, Heck AJR, Haberkant P, and Holthuis JC
J Lipid Res,, 59, 515-530 (2018)

Defective cortex glia plasma membrane structure underlies light-induced epilepsy in cpes mutants

Kunduri G, Turner-Evans D, Konya Y, Izumi Y, Nagashima K, Lockett S, Holthuis J, Bamba T, Acharya U, Acharya JK
Proc Natl Acad Sci USA, 115, E8919-E8928 (2018)

Colloidal Crystals of NaYF4 Upconversion Nanocrystals Studied by Small-Angle X-Ray Scattering (SAXS)

Homann C, Bolze J, Haase M
Part Part Syst Charact, 1800391 (2018)

Colloidal LaPO4:Gd\(^{3+}\) nanocrystals: X-ray induced single line UV emission

Espinoza S, Juestel T, Haase M
Nanoscale 10, 22533-22540 (2018)

Deep Ultraviolet Emitting Scintillators for Biomedical Applications: The Hard Way of Downsizing LuPO4:Pr\(^{3+}\)

Espinoza S, Volhard MF, Kätker H, Jenneboer H, Uckelmann A, Haase M, Müller M , Purschke M, Jüstel T
Part Part Syst Charact, 1800282 (2018)

NaYF4:Yb,Er/NaYF4 core/shell nanocrystals with high upconversion luminescence quantum yield

Homann C, Krukewitt L, Frenzel F, Grauel B, Würth C , Resch-Genger U, Haase M
Angew Chem Int Ed, 57, 8765–8769 (2018), DOI
Angew Chem, 130, 8901–8905 (2018), DOI

Holographic performance of silicon polymer films based on photoswitchable molecules

Bourdon B, Bock S, Kijatkin C, Shumelyuk A, Imlau M
Optical Material Express 8(7), 1951-1967 (2018)

Nonlinear Optical Potassium Niobate Nanocrystals as Harmonic Markers: The Role of Precursors and Stoichiometry in Hydrothermal Synthesis

Wang Z, Kijatkin C, Urban A, Haase M, Imlau M and Kömpe K
Nanoscale, 10, 10713-10720 (2018)

2017

Ligand-induced type II interleukin-4 receptor dimers are sustained by rapid re-association within plasma membrane microcompartments

Richter D, Moraga I, Winkelmann H, Birkholz O, Wilmes S, Schulte M, Kraich M, Kenneweg H, Beutel O, Selenschik P, Paterok D, Gavutis M, Schmidt T, Garcia KC, Müller TD, Piehler J
Nat Commun, 8, 15976 (2017)

Magnetic control of cellular processes using biofunctional nanoparticles

Monzel C, Vicario C, Piehler J, Coppey M, Dahan M
Chemical science, 8, 7330-7338 (2017)

Engineered Ferritin for Magnetogenetic Manipulation of Proteins and Organelles Inside Living Cells

Liße D, Monzel C, Vicario C, Manzi J, Maurin I, Coppey M, Piehler J, Dahan M
Advanced materials, 29, 1700189 (2017)

Surrogate Wnt agonists that phenocopy canonical Wnt and beta-catenin signalling

Janda CY, Dang LT, You C, Chang J, de Lau W, Zhong ZA, Yan KS, Marecic O, Siepe D, Li X, Moody JD, Williams BO, Clevers H, Piehler J, Baker D, Kuo CJ, Garcia KC
Nature, 545, 234-237 (2017)

Binding of interferon reduces the force of unfolding for interferon receptor 1

Chuartzman SG, Nevo R, Waichman S, Shental D, Piehler J, Levy Y, Reich Z, Kapon R
PLoS One, 12, e0175413 (2017)

STAT2 is an essential adaptor in USP18-mediated suppression of type I interferon signaling

Arimoto KI, Löchte S, Stoner SA, Burkart C, Zhang Y, Miyauchi S, Wilmes S, Fan JB, Heinisch JJ, Li Z, Yan M, Pellegrini S, Colland F, Piehler J, Zhang DE
Nature structural & molecular biology, 24, 279-289 (2017)

ER residency of the ceramide phosphoethanolamine synthase SMSr relies on homotypic oligomerization mediated by its SAM domain

Cabukusta B, Kol M, Kneller L, Hilderink A, Bickert A, Mina JG, Korneev S, Holthuis JC
Sci Rep, 7, 41290 (2017)

Diverting CERT-mediated ceramide transport to mitochondria triggers Bax-dependent apoptosis

Jain A, Beutel O, Ebell K, Korneev S, and Holthuis JC
J Cell Sci 130, 360-371 (2017)

Switching head group selectivity in mammalian sphingolipid biosynthesis by active-site-engineering of sphingomyelin synthases

Kol M, Panatala R, Nordmann M, Swart L, van Suijlekom L, Cabukusta B, Hilderink A, Grabietz T, Mina JGM, Somerharju P, Korneev S, Tafesse FG, and Holthuis JC
J Lipid Res, 58, 962-973 (2017)

Nonlinear Diffuse fs-Pulse Reflectometry of Harmonic Upconversion Nanoparticles

Kijatkin C, Eggert J, Bock S, Berben D, Oláh L, Szaller Z, Kis Z and Imlau M
Photonics, 4(1), 11 (2017)

2016

Drosophila neprilysins control insulin signaling and food intake via cleavage of regulatory peptides

Hallier B, Schiemann R, Cordes E, Vitos-Faleato J, Walter S, Heinisch JJ, Malmendal A, Paululat A, Meyer H
Elife, (2016) Dec 6;5:e19430

Monitoring Changes in the Oligomeric State of a Candidate Endoplasmic Reticulum (ER) Ceramide Sensor by Single-molecule Photobleaching

Cabukusta B, Köhlen JA, Richter CP, You C, Holthuis JC
J Biol Chem, 291(47):24735–24746 (2016)

Functional protein micropatterning for drug design and discovery

You, C, and Piehler, J
Expert opinion on drug discovery, 11, 105-119 (2016)

Receptor dimer stabilization by hierarchical plasma membrane microcompartments regulates cytokine signaling

You C, Marquez-Lago TT, Richter CP, Wilmes S, Moraga I, Garcia KC, Leier A, Piehler J
Science advances, 2, e1600452 (2016)

Functional interplay between SA1 and TRF1 in telomeric DNA binding and DNA-DNA pairing

Lin J, Countryman P, Chen H, Pan H, Fan Y, Jiang Y, Kaur P, Miao W, Gurgel G, You C, Piehler J, Kad NM, Riehn R, Opresko PL, Smith S, Tao YJ, Wang H.
Nucleic acids research, 44, 6363-6376 (2016)

Bioinspired monolithic polymer microsphere arrays as generically anti-adhesive surfaces

Eichler-Volf A, Kovalev A, Wedeking T, Gorb EV, Xue L, You C, Piehler J, Gorb SN, Steinhart M
Bioinspiration & biomimetics, 11, 025002 (2016)

Engineered Upconversion Nanoparticles for Resolving Protein Interactions inside Living Cells

Drees C, Raj AN, Kurre R, Busch KB, Haase M, Piehler J
Angew Chem Int Ed Engl, 55, 11668-11672 (2016)

Single molecule super-resolution imaging of proteins in living Salmonella enterica using self-labelling enzymes

Barlag B, Beutel O, Janning D, Czarniak F, Richter CP, Kommnick C, Göser V, Kurre R, Fabiani F, Erhardt M, Piehler J, Hensel M
Scientific reports, 6, 31601 (2016)

Structure of the Tuberous Sclerosis Complex 2 (TSC2) N Terminus Provides Insight into Complex Assembly and Tuberous Sclerosis Pathogenesis

Zech R, Kiontke S, Mueller U, Oeckinghaus A, Kümmel D
J Biol Chem, 291, 20008 (2016)

The N-terminus of the floral Arabidopsis TGA transcription factor PERIANTHIA mediates redox-sensitive DNA-binding

Gutsche N and Zachgo S
PlosOne, 29; 11(4):e0153810 (2016)